Share results of nasal microbiome project.

• Processing reads - from reads to count table

• Cluster analysis (Applying GoM - Grades of Membership model)
  • Choosing number of clusters
  • GOM cluster memberships under different seeds
  • Grades of membership model finalized
  • Variance explained under different k
  • Clustering using hierarchical clustering
• Cluster characteristics
  • Stacked bar plot of abundance
  • Diversity
  • Explore sample cluster probabilities in relation to the PCA outliers
• Differential abudance analysis between two clusters
  • At the species level
  • At higher phylogenetic taxa level

• Applying GoM to HMP data Results

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• Shiny site for our exploratory data analysis http://138.197.10.90:3838/shiny/shinyapp/

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